Device

Part:BBa_K4375018:Design

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-29)


MiniNano for bacterial surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1055
    Illegal PstI site found at 1313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
    Illegal PstI site found at 1055
    Illegal PstI site found at 1313
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1055
    Illegal PstI site found at 1313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1055
    Illegal PstI site found at 1313
    Illegal NgoMIV site found at 118
    Illegal NgoMIV site found at 451
    Illegal AgeI site found at 869
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1655
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimised for production in E. coli.



Source

[1] INPNC was adapted from: BBa_K811003 [2] Hernot S, Unnikrishnan S, Du Z, Shevchenko T, Cosyns B, Broisat A, Toczek J, Caveliers V, Muyldermans S, Lahoutte T, Klibanov AL, Devoogdt N. Nanobody-coupled microbubbles as novel molecular tracer. J Control Release. 2012 Mar 10;158(2):346-53. doi: 10.1016/j.jconrel.2011.12.007. Epub 2011 Dec 16. PMID: 22197777; PMCID: PMC3294017. [3] Oliinyk, O.S., Shemetov, A.A., Pletnev, S. et al. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, 279 (2019). https://doi.org/10.1038/s41467-018-08050-8


References