Device
Part:BBa_K4375018:Design
Designed by: Zska Csorba Group: iGEM22_ELTE (2022-09-29)
MiniNano for bacterial surface display
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1055
Illegal PstI site found at 1313 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 29
Illegal PstI site found at 1055
Illegal PstI site found at 1313 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1055
Illegal PstI site found at 1313 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1055
Illegal PstI site found at 1313
Illegal NgoMIV site found at 118
Illegal NgoMIV site found at 451
Illegal AgeI site found at 869
Illegal AgeI site found at 1523
Illegal AgeI site found at 1655 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We codon optimised for production in E. coli.
Source
[1] INPNC was adapted from: BBa_K811003 [2] Hernot S, Unnikrishnan S, Du Z, Shevchenko T, Cosyns B, Broisat A, Toczek J, Caveliers V, Muyldermans S, Lahoutte T, Klibanov AL, Devoogdt N. Nanobody-coupled microbubbles as novel molecular tracer. J Control Release. 2012 Mar 10;158(2):346-53. doi: 10.1016/j.jconrel.2011.12.007. Epub 2011 Dec 16. PMID: 22197777; PMCID: PMC3294017. [3] Oliinyk, O.S., Shemetov, A.A., Pletnev, S. et al. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, 279 (2019). https://doi.org/10.1038/s41467-018-08050-8